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Publications

Since 2019

  1. Luo, J.†; Lam, W.†,*; Yu, D.; Chao; V., Zopfi, M.; Khoo, C.; Zhao, C.; Yan, S.; Liu, Z.; Li, X.D.; Zheng, C.; Zhai, Y.*; Ti, S.*  (2024) Tubulin acetyltransferases access and modify the microtubule luminal K40 residue via anchors in taxine-binding pockets. Nature Structural & Molecular Biology
     

  2. Dodd, D.†; Mechaussier, S.†; Yeyati, P.; McPhie, F.; Anderson, J.; Khoo, C.J.; Shoemark, A.; Gupta, D.K.; Attard, T.;  Zariwala, M.A.; Legendre, M.; Bracht, D.; Wallmeier, J.; Gui, M.; Fassad, M.R.; Parry, D.A.; Tennant, P.A.; Meynert, A.; Wheway, G.; Fares-Taie, L.; Black, H.A.; Mitri-Frangieh, R.; Faucon, C.; Kaplan, J.; Patel, M.; McKie, L.; Megaw, R.; Gatsogiannis, C.; Mohamed, M.A.; Aitken, S.; Gautier, P.; Reinholt, F.R.; Hirst, R.A.; O’Callaghan, C.; Heimdal, K.; Bottier, M., Escudier, E., Crowley, S.; Descartes, M.; Jabs, E.W.; Kenia, P.; Amiel, J.; Bacci, G.M.; Calogero, C.; Palazzo, V.; Tiberi, L.; Blümlein, U.; Rogers, A.; Wambach, J.A.; Wegner, D.J.; Fulton, A.B.; Kenna, M.; Rosenfeld, M.; Holm, I.A.;  Quigley, A.; Hall, E.A.; Murphy, L.C.; Cassidy, D.M.; von Kriegsheim, A.; Partnership, S.G.; Genomics England Research Consortium, Undiagnosed Diseases Network, Papon, J.F.; Pasquier, L.; Murris, M.S.; Chalmers, J.D.; Hogg, C.; Macleod, K.A.; Urquhart, D.S.; Unger, S.; Aitman, T.J.; Amselem, S.; Leigh, M.W.; Knowles, M.R.; Omran, H.; Mitchison, H.M.; Brown, A.; Marsh, J.A.; Welburn, J.P.I.; Ti, S.C.; Horani, A.; Rozet, J.M.; Perrault, I.*, Mill, P.* (2024) Ciliopathy patient variants reveal organelle-specific functions for TUBB4B in axonemal microtubules. Science, 384, 6694, PMID: 38662826
     

  3. Fu, G.; Yan, S.; Khoo, C.J.; Chao, V.C.; Liu, Z.; Mukhi, M.; Hervas, R.; Li, X.D.; Ti, S.C. (2023) Integrated regulation of tubulin tyrosination and microtubule stability by human α-tubulin isotypes. Cell Reports, 42 (6), PMID: 37379209
     
  4. Tan, D.S.; Cheung, S.L.; Gao, Y.; Weinbuch, M.; Hu, H.; Shi, L.; Ti, S.C.; Hutchins, A.P.; Cojocaru, V.; Jauch, R. (2023) The homeodomain of Oct4 is a dimeric binder of methylated CpG elements. Nucleic Acids Res., 51, 3, 1120-1138, PMID: 36631980
     
  5. Fukuyama, T.; Yan, L.; Tanaka, M.; Yamaoka, M.; Saito, K.; Ti, S.C.; Liao, C.C.; Hsia, K.C.; Maeda, Y.T.; Shimamoto Y. (2022) Morphological growth dynamics, mechanical stability, and active microtubule mechanics underlying spindle self-organization. PNAS, 119, 44, e2209053119, PMID: 36282919
     

  6. Ti, S.C. (2022) Reconstituting microtubules: A decades-long effort from building block identification to the generation of recombinant α/β-Tubulin. Front. Cell Dev. Biol., PMID: 35573669
     

  7. Wieczorek, M*; Ti, S.C.*; Urnavicius, L; Molloy, K.R.; Aher, A.; Chait, B.T.; Kapoor, T.M. (2021) “Biochemical reconstitutions reveal principles of human γ-TuRC assembly and function” J. Cell Biology, 220 (3): e202009146. PMID: 33496729. *Co-first author.
     

  8. Ti,S.C.*;Wieczorek,M.;Kapoor,T.M.*(2020)“Purification of affinity-tag free recombinant tubulin from insect cells” STAR Protocols, 1, 1, 10011. PMID: 32783031. *Co-corresponding author.
     

  9. Wieczorek,M*;Urnavicius,L*;Ti,S.C.,Molloy,K.R.;Chait,B.T.;Kapoor,T.M.(2020)“Asymmetric molecular architecture of the human γ-tubulin ring complex” Cell, 180, 165-175. PMID: 31862189.

 

Prior Joining HKU in 2019

  1. Ti, S.C.; Alushin, G.M.; Kapoor, T.M. (2018) “Human β-tubulin isotypes can regulate microtubule protofilament number and stability” Developmental Cell, 47, 175-190. PMID: 30245156.
     

  2. Kellogg, E.H.; Howes, S.; Ti, S.C.; Ramirez-Aportela, E.; Kapoor, T.M.; Chacón, P.; Nogales, E. (2016) “Near-atomic cryo-EM structure of PRC1 bound to the microtubule” PNAS, 113, 9430-9439. PMID: 27493215.
     

  3. Pamula,M.C.;Ti,S.C.;Kapoor,T.M(2016)“The structure core of human β-tubulin confers isotype- specific polymerization properties” J. Cell Biology, 213, 425-33. PMID: 27185835.
     

  4. Ti,S.C.;Pamula,M.C.;Howes,S.C.;Duellberg,C.;Cade,N.I.;Kleiner,R.E.;Forth,S.;Surrey,T.; Nogales, E.; Kapoor, T.M. (2016) “Mutations in human tubulin proximal to the kinesin-binding site alter dynamic instability at microtubule plus and minus ends” Developmental Cell, 37, 72-84. PMID: 27046833.
     

  5. McCormickC.D.;AkamatsuM.S.;TiS.C.;PollardT.D.(2013)“Measuring affinities of fission yeast spindle pole body proteins in live cells across the cell cycle” Biophysics J., 105, 1324-35. PMID: 24047983.
     

  6. Kleiner R.E.; Ti S.C.; Kapoor T.M. (2013) “Site-specific chemistry on the microtubule polymer” JACS, 135 (34), 12520-3. PMID: 23930594.
     

  7. Subramanian R.; Ti S.C.; Tan L.; Darst S.A.; Kapoor T.M. (2013) “Marking and measuring single microtubules by PRC1 and Kinesin-4” Cell, 154 (2): 377-90. PMID: 23870126.
     

  8. Ti S. C.*; Jurgenson C.*; Nolen, B. J.; Pollard, T. D. (2011) “Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex” PNAS, 108 (33): E463-71. PMID: 21676862
     

  9. Ti S. C.; Pollard, T.D. (2011) “Purification of actin from fission yeast Schizosaccharomyces pombe and characterization of functional differences from muscle actin” J. Biological Chemistry, 286 (7), 5784-92. PMID: 21148484.
     

  10. Cheng, C. H.; Lo, Y. H.; Liang, S. S.; Ti, S. C.; Lin, F. M.; Yeh, C. H.; Huang, H. Y.; Wang, T. F. (2006) “SUMO modifications control assembly of synaptonemal complex and polycomplex in meiosis of Saccharomyces cerevisiae” Genes and Development 20, 1986-1992. PMID:16847351.
     

  11. Chen, Y. K.; Leng, C. H.; Olivares, H.; Lee, M. H.; Chang. Y. C.; Kung, W. M.; Ti, S. C.; Lo, Y. H.; Wang, A. H.; Chang C. S.; Bishop D. K.; Hsueh Y. P.; Wang, T. F. (2004) “Heterodimeric complexes of meiosis-specific Hop2 and Mnd1 promote DNA recombination and homolog juxtaposition” PNAS 101, 10572-77. PMID: 15249670.

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